Here's how you would add the ListViewItemcode you created to yours ListView:
myListView.Items.Add(new ListViewItem { Content = "This is an item added programmatically." });
However, I agree with MrTelly that this is not necessary, you should install ListView.ItemsSourcein some collection, and not directly manipulate ListView.Items.
If you give us more detailed information about what you want to accomplish, maybe we can help you do it in WPF way, which is not always easy, but in the long run it is much easier.