Short answer: given that you are writing to a Flash drive, I would not expect the number of threads to make a big difference anyway. But if that mattered, I would expect multiple threads to be faster than one thread, not slower.
Longer answer:
I wrote a similar program to what you described about 6 years ago - it ran on the built-in PowerPC Linux card and read / wrote several simultaneous audio files to the SCSI hard drive. I originally wrote it using a single thread doing I / O, because I thought this would give better throughput, but it turned out that it wasn’t.
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