First, you should read the docs events well here .
You can attach a function that is called with every mouse click. If you maintain a list of artists (dots in this case) that you can select, you can ask if there was a mouse click event inside the artists, and call the artist's remove method. If not, you can create a new artist and add it to the list of clicked points:
import matplotlib.pyplot as plt fig = plt.figure() ax = plt.axes() ax.set_xlim(0, 1) ax.set_ylim(0, 1) pickable_artists = [] pt, = ax.plot(0.5, 0.5, 'o') # 5 points tolerance pickable_artists.append(pt) def onclick(event): if event.inaxes is not None and not hasattr(event, 'already_picked'): ax = event.inaxes remove = [artist for artist in pickable_artists if artist.contains(event)[0]] if not remove: # add a pt x, y = ax.transData.inverted().transform_point([event.x, event.y]) pt, = ax.plot(x, y, 'o', picker=5) pickable_artists.append(pt) else: for artist in remove: artist.remove() plt.draw() fig.canvas.mpl_connect('button_release_event', onclick) plt.show()

Hope this helps you achieve your dream. :-)
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