The boot sector of a floppy disk was the first sector. If you're talking about a raw floppy disk (1440K), this should be the first 512 bytes of the image file.
From memory, this is loaded by the BIOS at 7c00: 0000 (real mode), and then goes to that address.
DOS boot floppies had a 3-byte JMP command to jump over the disk parameter block (DPB), which details disk attributes. But if you have full control of the drive and your boot code, I donβt think you need to follow this convention. I donβt remember any BIOS checking what was loaded for reality (although admittedly, it was a long time ago).
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