After doing the research, I found the problem, because yes, 0.2 seconds slower.
SELECT t.trip_headsign, st.stop_sequence, s.stop_code, s.stop_name FROM stop_times AS st JOIN stops AS s USING (stop_id) JOIN ( SELECT trip_id, route_id, trip_headsign FROM trips WHERE route_id = '141' LIMIT 2 ) AS t WHERE t.trip_id = st.trip_id GROUP BY st.stop_id
First, instead of executing a LEFT JOIN here JOIN is faster. But an important point I compared all the results of the trips in the WHERE instruction.
However, since the bus can have only 2 directions, I need to limit my results to 2. Now my results are approaching 0.018. Over 1000% improvement.
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