If your string is defined as:
string str = @"line1
line2
line3
line4";
Then you can do:
string newStr = str.Substring(0, str.LastIndexOf(Environment.NewLine));
If your line has a start / end space or line break, you can do:
string newStr = str
.Substring(0, str.Trim().LastIndexOf(Environment.NewLine));