Use a list comprehension and list.count:
>>> ls = [1, 2, 3, 3]
>>> [x for x in ls if ls.count(x) == 1]
[1, 2]
>>>
.
Edit:
@ . , .
:
>>> from collections import Counter
>>> ls = [1, 2, 3, 3]
>>> c = Counter(ls)
>>> [x for x in ls if c[x] == 1]
[1, 2]
>>>
collections.Counter.