You need to make sure that your language settings support the character you want to use, and that the file is saved with the correct encoding. Saving as UTF-8 worked for me.
I was able to reproduce your situation in Windows 7 and tried a bunch of things. Nested in Rmarkdown, here is a minimal working example.
```{r}
Sys.setlocale("LC_ALL","German")
```
```{r}
Encoding("Título")
library(dygraphs)
dygraph(AirPassengers, main = "Título")
```
, , Encoding(). , , , "Título" , , , javascript dygraph. UTF-8 <U+00ED>, javascript, , . utf-8, @Michele, .
, , html/javascript, . , , title . - Rstudio "Viewer", javascript, , .