On the page and readme, py2app aims to create Mac applications for python code. Obviously, you cannot run the final product on a Windows machine. But if the question is whether you can create an application on Windows and run it on a Mac, then there is still no answer, because if you go to setup.py , it says 'Operating System :: MacOS :: MacOS X' , and there are no forks for windows, because it depends on some Mac SDK tools, such as /Developer/SDKs/MacOSX10.5.sdk . You seem unlucky. If you are desperate, try Virtual Block to emulate the Mac on your Windows machine or be happy with creating windows using py2exe
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