First, create two data samples with the same names as in the example.
dat<-data.frame(ve=rep(c("FF","GG"),times=50), metValue=rnorm(100),metric=rep(c("A","B","D","C"),each=25), atd=rep(c("HH","GG"),times=50)) dat2<-data.frame(ve=rep(c("FF","GG"),times=50), metValue=rnorm(100),metric=rep(c("A","B","D","C"),each=25), atd=rep(c("HH","GG"),times=50))
I assume that you do not need to use the fill= argument in geom_jitter() , because the color for shape=4 can also be set with the colour= argument. Then you can use scale_colour_manual() to set your values. Instead of cpallete , only color names are used.
P <- ggplot(dat) + geom_boxplot(aes(x=ve, y=metValue, fill=metric), alpha=.35, w=0.6, notch=FALSE, na.rm = TRUE) + geom_hline(yintercept=0, colour="#DD4466", linetype = "longdash") + scale_fill_manual(values=c("red","blue","green","yellow"))+ theme(legend.position="none") P + geom_jitter(data=dat2, aes(x=ve, y=metValue, colour=atd), size=2, shape=4, alpha = 0.4, position = position_jitter(width = .03, height=0.03), na.rm = TRUE) + scale_colour_manual(values=c("red","blue"))
