It's a shot in the dark because I don't know anything about serving audio files in iOS, but in case it helps ...
Around the same time that you submitted this question, a friend asked me to help him with his site. The website previously played audio, but during your question the sound stopped working in Safari on OS X.
The existing audio player that was used was based on Flash. I switched my website to playing audio using HTML5, and then it worked fine.
So, you can check if you are playing audio in iOS using a Flash player and you can change it to HTML5 if you can.
Jeff o'neill
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