Short answer: no
I recently did something similar (using cx_Freeze with Python 3). If you configure Python inside Wine, you can create a Windows assembly, but I had to copy some DLLs before it worked correctly (cx_Freeze calls a Windows API function that is not implemented in Wine). I have not come across any way of packing applications for Mac computers without actually using a Mac.
Perhaps someone should set up a community build service so that people can create distributions for different platforms for each other. However, this does not pose a testing problem.
Thomas K
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