VS 2008 / AJAX project does not work live

I have been working on the VB.NET/VS2008/AJAX/SQL Server project for more than two years without any real problems. However, we are in the last week of our project, conducting heavy stress tests, and the project starts to crash as soon as I get about 150 concurrent users. I even went so far as to create a truncated version of the site, which is only registered by the user, pulls out my profile and then logs out. It still does not withstand stress. When I say "crash", I mean that the CPU has spikes, and ultimately the application package crashes. It runs on a quad-core Windows 2008 R2 server with 16 gigabytes of memory. Memory never accumulates, but the processor runs out.

I ran YSlow on the site, and it indicated that I need to compress .axd files, etc. I did this by implementing Gzip compression in everything, but what attracted me to 150 users. Now I run YSlow and it says that everything is "A".

I'm really not sure where to go from here. I would be more than willing to share a split version of the site for anyone to consider. I am not sure if it is a server, my code or web.config.

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I know it's a little late, but do you think you are increasing the number of workflows in your site’s application pool to form a web garden? This can be done in IIS Manager.

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