If I use mp3: "/Music/StreamUploadedSongs/1" in the following code:
var player = new $("#jquery_jplayer_1").jPlayer({ ready: function () { $(this).jPlayer("setMedia", { mp3: "/Music/StreamUploadedSongs/1", }); }, cssSelectorAncestor: "#jp_container_1", swfPath: "~Scripts/Jplayer/jquery.jplayer.swf", useStateClassSkin: true, autoBlur: false, keyEnabled: true } });
Here's what it looks like: you can see that jplayer is not moving the time correctly (they overlap), and also the search / playback panel does not work, although the song can still play: 
Html Markup: <audio id="jp_audio_0" preload="metadata" src="http://localhost:6060/Music/StreamUploadedSongs/1"></audio>
Music Controller:
public ActionResult StreamUploadedSongs(int id) { byte[] song = db.UploadedSongs.Where(x => x.Id == id).FirstOrDefault().SongBytes; return File(song, "audio/*"); }
If instead change the mp3: mp3: "http://www.jplayer.org/audio/mp3/TSP-01-Cro_magnon_man.mp3" property mp3: "http://www.jplayer.org/audio/mp3/TSP-01-Cro_magnon_man.mp3" , then it works great.
var player = new $("#jquery_jplayer_1").jPlayer({ ready: function () { $(this).jPlayer("setMedia", { mp3: "http://www.jplayer.org/audio/mp3/TSP-01-Cro_magnon_man.mp3", }); }, cssSelectorAncestor: "#jp_container_1", swfPath: "~Scripts/Jplayer/jquery.jplayer.swf", useStateClassSkin: true, autoBlur: false, keyEnabled: true }
});
Here's what it looks like, working correctly, the arrow / playback bar works, and jplayer moved the time to the correct positions: 
Html Markup: <audio id="jp_audio_0" preload="metadata" src="http://www.jplayer.org/audio/mp3/TSP-01-Cro_magnon_man.mp3"></audio>
I have other jPlayers on other pages as well as the same with them.
Edit : just tried this:
public string StreamUploadedSongs(int id) { string filePath = Server.MapPath(Url.Content("~/Content/TSP-01-Cro_magnon_man.mp3")); return filePath; }
I put the mp3 file in this directory, but now it does not play at all. If I paste this into the url http://localhost:6060/Music/StreamUploadedSongs/1034" , it just returns I: \ Users \ UserName \ Desktop \ MusicSite \ MusicSite \ MusicSite \ Content \ TSP-01-Cro_magnon_man.mp3 instead of playing songs.